Reference ID | 7047 | ||||
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Title | Subcellular localization of proteins listed in Gramene database | ||||
Source | Gramene database, 2002, vol. 1, pp. 1-1 | ||||
Authors (3) |
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Abstract | In Gramene database, the localization of protein to different subcellular components in a plant cell are based on the suggestions or inferences derived from, (1) peer reviewed articles associated with the respective protein, (2) determined by sequence similarity (ISS) in absence of a published article, (3) Prediction analyses using locally installed copy of the most recent version of Predotar (available from http://www.inra.fr/predotar/). More details on the program used for prediction is available from http://www.inra.fr/predotar/interpretation.html, (4) Prediction analyses using web based tool TargetP, available at http://www.cbs.dtu.dk/services/TargetP/. Ref: Emanuelsson et. al. (2000), Predicting subcellular localization of proteins based on their N-terminal amino acid sequence", J. Mol. Biol. 300, 1005-1016. Parameters for TargetP predictions are (a) Cleavage site predictions included, (b) Using PLANT networks and (c) cutoff score take all, cTP/0.00 mTP/0.00 SP/0.00 other/0.00. (5) Inferred by curator (IC), which is used for those cases where an annotation is not supported by any evidence, but can be reasonably inferred by a curator from other gene ontology (GO) annotations, for which an evidence is available. All the associations carry with them an evidence listed either in the Gramene literature database or hyper linked to an external source. The associated reference is supported with an evidence code, suggesting the type of experiment/assertions used in inferring the association. The evidence codes are listed at http://www.gramene.org/plant_ontology/evidence_codes.html. |
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