Reference "Mapping quantitative trait loci controlling seed longevity in rice ( Oryza sativa L.)"

Reference ID

7749

Title

Mapping quantitative trait loci controlling seed longevity in rice ( Oryza sativa L.)

Source

Theoretical and applied genetics, 2002, vol. 104, pp. 981-986

Authors (4)

Miura-K	Lin-Y
Yano-M	Nagamine-T

Abstract

Quantitative trait loci (QTLs) controlling seed longevity in rice were
identified using 98 backcross inbred lines (BILs) derived from a cross between a
japonica variety Nipponbare and an indica variety Kasalath. Seeds of each BIL
were kept for 12 months at 30 degrees C in dry conditions to promote loss of
viability. To measure seed longevity, we performed an additional aging-
processing treatment for 2 months at 30 degrees C maintaining seeds at 15%
moisture content. We measured the germination percent of these treated seeds at
25 degrees C for 7 days as the degree of seed longevity. The germination of BILs
ranged from 0 to 100% with continuous variation. Three putative QTLs for seed
longevity, qLG-2, qLG-4 and qLG-9, were detected on chromosome 2, 4 and 9,
respectively. Kasalath alleles increased the seed longevity at these QTLs. The
QTL with the largest effect, qLG-9, explained 59.5% of total phenotypic
variation in BILs. The other two QTLs, qLG-2 and qLG-4, explained 13.4 and 11.6%
of the total phenotypic variation, respectively. We also verified the effect of
the Kasalath allele of qLG-9 using chromosome segment substitution lines.
Furthermore, QTLs for seed dormancy were identified on chromosomes 1, 3, 5, 7
and 11. Based on the comparison of the chromosomal location of QTLs for seed
longevity and seed dormancy, these traits seem to be controlled by different
genetic factors.