Reference "Mapping quantitative trait loci associated with rice grain shape based on an indica/japonica backcross population"

Reference ID

8185

Title

Mapping quantitative trait loci associated with rice grain shape based on an indica/japonica backcross population

Source

Acta genetica Sinica, 2003, vol. 30, pp. 711-716

Authors (9)

Yan-C-J	Liang-G-H
Chen-F	Li-X
Tang-S-Z	Yi-C-D
Tian-S	Lu-J-F
Gu-M-H

Abstract

Appearance of rice grain represents a major character of rice quality in many
rice-producing areas of the world, especially in hybrid rice production in
China. In this study, we conducted a molecular marker-based genetic analysis of
the traits that are determinants of the appearance quality of rice grains,
including grain length, grain width and grain shape (measured as grain length to
grain width ratio). Two typical indica/japonica varieties Balilla and
Nantehao(NTH) were selected to construct Balilla/NTH//Balilla backcross
population containing 142 individuals. In the population, grain length, grain
width and grain shape all conform to the normal distribution with certain
transgressive segregation. It can be deduced that all of three traits were
controlled by some quantitative trait loci (QTLs). In order to explore the QTLs
effect, number and location, a linkage map consisting of 108 SSR markers based
on the backcross population was constructed, and QTLs mapping was carried out
for grain length, grain width and grain shape. A QTL, qGL-12, was detected for
grain length at the interval RM101-RM270 on chromosome 12, its additive effect
was 0.26 mm, and can explain 16.7% genetic variation. As for grain width trait,
two QTLs were found, qGW-2 located at RM154-RM211 interval on chromosome 2, and
qGW-3 at interval RM257-RM175 on chromosome 3, accounting for 11.5% and 16.6%
genetic variation, respectively. The alleles at qGW-2 and qGW-3 from parent
Balilla can increase grain width by 0.10 mm and 0.12 mm. For grain shape, 3
QTLs, qLW-2, qLW-6 and qLW-7 were found, located on chromosome 2, 6, and 7,
respectively. qLW-2 and qLW-7 had positive effect, and they can explain 12.7%
and 18.3% genetic variation, while qLW-6 had negative effect and contributed
11.5% genetic variation to the backcross population. The prospects of
application of linkage relationship between SSR marker and QTLs in marker based
selection (MAS) in rice breeding, and the improvement of grain shape and rice
appearance quality were discussed.