Reference "Genetic bases of instability of male sterility and fertility reversibility in photoperiod-sensitive genic male-sterile rice"

Reference ID

7960

Title

Genetic bases of instability of male sterility and fertility reversibility in photoperiod-sensitive genic male-sterile rice

Source

Theoretical and applied genetics, 1999, vol. 99, pp. 683-693

Authors (5)

He-Y-Q	Yang-J
Xu-C-G	Zhang-Z-G
Zhang Q

Abstract

Abstract Photoperiod-sensitive genetic male-sterile (PSGMS) rice, with its male
fertility regulated by photoperiod length, is very useful for hybrid rice
development. However, breeding for new PSGMS lines has faced two major
difficulties - the stability of male sterility and the reversibility of male
fertility. In this study we assessed the genetic bases of stability of sterility
and fertility reversibility using a molecular marker-based approach. A cross was
made between two newly bred PSGMS lines: Peiai 64S, which has a stable sterility
but is difficult to reverse to fertility, and 8902S, which has a unstable
sterility but is easy to reverse to fertility. The fertility of the parents and
of the F1 and F2 populations was repeatedly examined under 11 different long-day
and short-day conditions. The genetic effects were assayed by interval mapping
and two-way analyses of variance using the F2 data of 128 polymorphic loci
representing all the 12 rice chromosomes. The analyses resolved a number of single-
locus QTLs and two-locus interactions under both long-day and short day
conditions. The interactions involved a large number of loci, most of which were
not detectable on a single-locus basis. The results showed that the genetic
bases of both stability of sterility and reversibility of fertility are the
joint effects of the additive effects of the QTLs and additive-by-additive
components of two-locus interactions. The implications of these findings in
hybrid rice development are also discussed.