Reference "Quantitative trait locus mapping of ovicidal response in rice (Oryza sativa L.) against whitebacked planthopper (Sogatella furcifera Horvath)"

Reference ID

7918

Title

Quantitative trait locus mapping of ovicidal response in rice (Oryza sativa L.) against whitebacked planthopper (Sogatella furcifera Horvath)

Source

Crop Science, 1999, vol. 39, pp. 1178-1183

Authors (5)

Tsunematsu-H	Yasui-H
Yoshimura-A	Iwata-N
Yamasaki-M

Abstract

The ovicidal response in rice (Oryza sativa L.) to whitebacked planthopper
(WBPH), Sogatella furcifera Horvath, is characterized by the formation of watery
lesions which result in the death of WBPH eggs. To determine the genetic
mechanism of this ovicidal response, a set of 71 rice recombinant inbred (RI)
lines (F(8), F(9), and F(10)) derived from a cross of japonica cultivar
Asominori and indica cultivar IR24, the two parents, and the F(1) were
phenotyped for percentage of watery lesions (PWL) and WBPH egg mortality (EM).
PWL and EM showed significant positive correlations (P < 0.001) and
transgressive segregation was observed for both traits. A total of 10
quantitative trait loci (QTLs) for ovicidal response were detected with 292 RFLP
markers in the F(6) and F(7) populations by composite interval mapping (LOD > or
= to 1.5). Four of 10 QTLs coincided for PWL and EM and each three QTLs were
only detected for PWL and EM. The QTL on chromosome 6 (R1954-L688) was most
significantly associated with the ovicidal response and accounted for 69.9% of
phenotypic variance for PWL (F(8)) and 46.0% of phenotypic variance for EM
(F(8)). Positive alleles for ovicidal response came from Asominori on
chromosomes 1, 3, 6S (short arm), 8, and 12 and from IR24 on chromosomes 2, 6L
(long arm), and 10. QTL accumulation from both parents was the genetic basis of
the transgressive segregation in the RI population. No epistatic interaction was
detected. The Asominori allele on the chromosome 6S QTL was essential to the
ovicidal response, and R1954 was found to be a target marker for marker assisted
selection.