Reference "Saturated molecular map of the rice genome based on an interspecific backcross population"

Reference ID

456

Title

Saturated molecular map of the rice genome based on an interspecific backcross population

Source

Genetics, 1994, vol. 138, pp. 1251-1274

Authors (13)

Causse-M-A	Fulton-T-M	Cho-Y-G	Ahn-S-N
Chunwongse-J	Wu-K	Xiao-J	Yu-Z
Ronald-P-C	Harrington-S-E	Second-G	McCouch-S-R
Tanksley-S-D

Abstract

A molecular map has been constructed for the rice genome comprised of 726
markers (mainly restriction fragment length polymorphisms; RFLPs). The mapping
population was derived from a backcross between cultivated rice, Oryza sativa,
and its wild African relative, Oryza longistaminata. The very high level of
polymorphism between these species, combined with the use of polymerase chain
reaction-amplified cDNA libraries, contributed to mapping efficiency. A subset
of the probes used in this study was previously used to construct an RFLP map
derived from an inter subspecific cross, providing a basis for comparison of the
two maps and of the relative mapping efficiencies in the two crosses. In
addition to the previously described PstI genomic rice library, three cDNA
libraries from rice (Oryza), oat (Avena) and barley (Hordeum) were used in this
mapping project. Levels of polymorphism detected by each and the frequency of
identifying heterologous sequences for use in rice mapping are discussed. Though
strong reproductive barriers isolate O. sativa from O. longistaminata, the
percentage of markers showing distorted segregation in this backcross population
was not significantly different than that observed in an intraspecific F2
population previously used for mapping. The map contains 1491 cM with an average
interval size of 4.0 cM on the framework map, and 2.0 cM overall. A total of 238
markers from the previously described PstI genomic rice library, 250 markers
from a cDNA library of rice (Oryza), 112 cDNA markers from oat (Avena), and 20
cDNA markers from a barley (Hordeum) library, two genomic clones from maize
(Zea), 11 microsatellite markers, three telomere markers, eleven isozymes, 26
cloned genes, six RAPD, and 47 mutant phenotypes were used in this mapping
project. Applications of a molecular map for plant improvement are discussed.