Reference "Mapping quantitative trait loci underlying appearance quality of rice grains (Oryza sativa L.)"

Reference ID

7967

Title

Mapping quantitative trait loci underlying appearance quality of rice grains (Oryza sativa L.)

Source

Acta genetica Sinica, 2003, vol. 30, pp. 251-259

Authors (4)

Li-Z-F	Wan-J-M
Xia-J-F	Zhai-H-Q

Abstract

Ninety-eight backcross inbred lines (BILs) derived from a subspecific cross of
Nipponbare (japonica)/Kasalath (indica)//Nipponbare and its genetic linkage map
were used to identify quantitative trait locus (QTL) controlling rice appearance
quality traits such as grain length (GL), grain width (GW), length-width ratio
(LWR), percentage of grains with chalkiness (PGWC), square of chalky endosperm
(SCE), degree of endosperm chalkiness (DEC) and grain endosperm transparency
(GET) by composite interval mapping over two years. A total of 33 QTLs were
identified to be significant in at least one year for seven traits and the
number of QTL for each trait ranged from four to seven, showing that appearance
quality of rice grains were controlled by multigenes. The phenotypic variation
explained by individual QTLs ranged from 6.2% to 15.2% for GL, 8.3% to 32.5% for
GW, 6.8% to 19.8% for LWR, 6.4% to 28.5% for PGWC, 6.1% to 16.9% for SCE, 9.3%
to 17.2% for DEC, and 5.6% to 25.2% for GET. The distribution of QTL were
characterized by clustering with the intervals of C1488-C563 on chromosome 3,
R830-R3166 and R1436-R2289 on chromosome 5, R2147-R2171 on chromosome 6 each
harboring over 3 QTLs. The QTL-by-environment interactions were observed by
comparing QTL mapping of the same population grown in 2 consecutive years, but
were trait dependent. The QTL for GL and LWR were rather stable across years
while that for PGWC, SCE and DEC were very sensitive to environments.