Reference "RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper ( Nilaparvata lugens)"

Reference ID

7744

Title

RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper ( Nilaparvata lugens)

Source

Theoretical and applied genetics, 2002, vol. 104, pp. 248-253

Authors (5)

Xu-X-F	Mei-H-W
Luo-L-J	Cheng-X-N
Li-Z-K

Abstract

Quantitative trait loci (QTLs), conferring quantitative resistance to rice brown
planthopper (BPH), were investigated using 160 F(11) recombinant inbred lines
(RILs) from the Lemont/Teqing cross, a complete RFLP map, and replicated
phenotyping of seedbox inoculation. The paternal indica parent, Teqing, was more-
resistant to BPH than the maternal japonica parent, Lemont. The RILs showed
transgressive segregation for resistance to BPH. Seven main-effect QTLs and many
epistatic QTL pairs were identified and mapped on the 12 rice chromosomes.
Collectively, the main-effect and epistatic QTLs accounted for over 70% of the
total variation in damage scores. Teqing has the resistance allele at four main-
effect QTLs, and the Lemont allele resulted in resistance at the other three. Of
the main-effect QTLs identified, QBphr5b was mapped to the vicinity of gl1, a
major gene controlling leaf and stem pubescence. The Teqing allele controlling
leaf and stem pubescence was associated with resistance, while the Lemont allele
for glabrous stem and leaves was associated with susceptibility, indicating that
this gene may have contributed to resistance through antixenosis. Similar to the
reported BPH resistance genes, the other six detected main-effect QTLs were all
mapped to regions where major disease resistance genes locate, suggesting they
might have contributed either to antibiosis or tolerance. Our results indicated
that marker-aided pyramiding of major resistance genes and QTLs should provide
effective and stable control over this devastating pest.