Reference "Isolation of rice genes possibly involved in the photoperiodic control of flowering by a fluorescent differential display method"

Reference ID

7059

Title

Isolation of rice genes possibly involved in the photoperiodic control of flowering by a fluorescent differential display method

Source

Plant & cell physiology, 2002, vol. 43, pp. 494-504

Authors (3)

Hayama-R	Izawa-T
Shimamoto-K

Abstract

To better understand the molecular mechanisms of the photoperiodic regulation of
rice, a short-day plant, we isolated 27 cDNAs that were differentially expressed
in the photoperiod-insensitive se5 mutant from approximately 8,400 independent
mRNA species by the use of a fluorescent differential display (FDD). For this
screening, we isolated mRNAs at five different time points during the night and
compared their expression patterns between se5 and the wild type. Of 27 cDNAs
isolated, 12 showed diurnal expression patterns often associated with genes
involved in the determination of the flowering time. In se5, expression of nine
cDNAs was increased. Five of these cDNAs were up-regulated under SD, suggesting
that they may promote flowering under SD. They included genes encoding a cDNA
containing a putative NAC domain, the fructose-bisphosphate aldolase, and a
protease inhibitor. Expression of three cDNAs was decreased in se5 but not
photoperiodically regulated. These cDNAs included a rice homolog of Arabidopsis
GIGANTEA (GI), lir1, and a gene for myo-inositol 1-phosphate synthase, all of
which were previously shown to be under the control of circadian clocks. The
expression patterns of the rice homolog of GI, OsGI, were similar to those of
the Arabidopsis GI, suggesting the conservation of some mechanisms for the
photoperiodic regulation of flowering between these two species.