Reference "A mutant of rice lacking the leaf large subunit of ADP-glucose pyrophosphorylase has drastically reduced leaf starch content but grows normally"

Reference ID

25473

Title

A mutant of rice lacking the leaf large subunit of ADP-glucose pyrophosphorylase has drastically reduced leaf starch content but grows normally

Source

Functional Plant Biology, 2007, vol. 34, pp. 480-489

Authors (3)

Rösti-S	Fahy-B
Denyer-K

Abstract

A mutant of rice was identified with a Tos17 insertion in OsAPL1, a gene
encoding a large subunit (LSU) of ADP-glucose pyrophosphorylase (AGPase). The
insertion prevents production of a normal transcript from OsAPL1.
Characterisation of the mutant (apl1) showed that the LSU encoded by OsAPL1 is
required for AGPase activity in rice leaf blades. In mutant leaf blades, the
AGPase small subunit protein is not detectable and the AGPase activity and
starch content are reduced to <1 and <5% of that in wild type blades,
respectively. The mutation also leads to a reduction in starch content in the
leaf sheaths but does not significantly affect AGPase activity or starch
synthesis in other parts of the plant. The sucrose, glucose and fructose
contents of the leaves are not affected by the mutation. Despite the near
absence of starch in the leaf blades, apl1 mutant rice plants grow and develop
normally under controlled environmental conditions and show no reduction in
productivity.