Vol. 20 >B. Research Notes>IV. Genetics of physiological traits and others |
16. | The amylopectin chains length distribution of opaque endosperm type in Myanmar local rice cultivar |
PA PA AUNG, A. NISHI, T. KUMAMARU and H. SATOH Plant Genetics Laboratory, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, 812 Japan |
Gelatinization property is one of the most important rheological indicators
of cooking quality and processing characteristic of rice starch. Amylopectin
side chains length affects the gelatinization of rice endosperm starch
granules in urea solution as well as in alkali solution (Nishi et al.,
2001, Umemoto et al., 2002). Hanashiro et al., (1996) reported
that the amylopectin chain with DP <= 12, 13 <= DP <= 24, 25
<= DP <= 36 and DP >= 37 correspond to A chains, B1
chains, B2 chains and long chains of amylopectin, respectively.
Variations of the amylopectin chains length structure have been observed
in dull, ae, sbe1, sbe2, sug1, sug2
and flo2 endosperm of rice mutant lines (Okuno et al., 1983,
Nishi et al., 2001, Kubo et al., 1999, and Satoh et al.,
2003). However, no report on the amylopectin chains length structure of
opaque endosperm in rice has been done. This is the first report dealing
with the amylopectin chains length distribution of opaque endosperm type
in Myanmar local rice cultivar. 11, and slightly increase in B1 chain with DP 19- 36 and in long chains with DP >= 37 (Fig. 2 C and D). The A chains proportion fa (DP <= 12) of MMR310 showed lower than that of Kinmaze and higher than that of IR36, while B1 chains fb1 (13 <= DP <= 24) had higher than that of Kinmaze and lower than that of IR36. The difference in the proportion of fb2 (25 <= DP <= 36) was not significant among three varieties. On the other hand, the differences in the proportions of fb3 in IR36 and MMR310 showed higher than that of Kinmaze (Table 1). The activity of starch synthase IIa affects the proportion of short chains (Umemoto et al., 2002). This shows the higher proportion of short chains, therefore the activity levels of SSIIa in MMR310 are estimated lower than that of IR36 and higher than that of Kinmaze. MMR310 had significantly increase in short chains compared with that of IR36, while remarkably decrease in short chains compared with that of Kinmaze, suggesting that the amylopectin structure of MMR310 was different between IR36 and Kinmaze. The B1 chains and long chains amylopectin structure of MMR310 were distinct from that of Kinmaze and IR36 and the proportion of A chains of MMR310 showed between IR36 and Kinmaze. The amylopectin fine structure of opaque endosperm type was different from that of Kinmaze and IR36, suggesting that the different physicochemical properties of starch and amylopectin fine structures of opaque starches depended on the value of alkali digestibility. Therefore, the value of alkali digestibility or gela- tinization temperature in rice is very important for determining
of physicochemical starch properties and the fine structure of amylopectin. |
Vol. 20 >B. Research Notes>IV. Genetics of physiological traits and others |