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15. | Repeating backcrosses for induced earliness gene, Ef1-x |
S.H. CHANG 1, M.S. CHANG 1, N.T. HU 3
and K.H. TSAI 2 1) Graduate Institute of Agronomy, National Chung-Hsing University, Taichung, Taiwan, ROC 2) Department of Agronomy, National Chung-Hsing University, Taichung, Taiwan, ROC 3) Biotechnology Institute, National Chung-Hsing University, Taichung, Taiwan, ROC |
Tsai and Oka (1965, 1968) identified Ef1, a dominant gene for
early-heading. It accelerates floral initiation by about 10 days in the
genetic background of T65 (Taichung 65 from Taiwan) carrying the recessive
allele, ef1. The different Ef1 alleles were considered to
be isoalleles, and the near-isogenic lines of T65 with these Ef1
alleles were established (Tsai 1976). The early heading mutant of T65,
T65(0)Ex ((0)Ex), with Ef1-x gene was obtained
by X-ray irradiation (Tsai 1971). The (0)Ex line was backcrossed
to T65 five times and (5)Ex line was obtained. The backcrossing
seemed to weaken the effects of the earliness relative to the other Ef1
lines tested. Thus, the backcrosses were further increased to nine times
to purify the Ef1-x gene, and (9)Ex was obtained (Tsai
1988). for the earliness gene carried in line (9)Ex to reduce height.
The estimated effective factors for heading time are 1.81 by using a hybrid
between (0)Ex and (9)Ex lines .
It suggested that x-ray irradiation might have induced ef1 domain
to show earliness behavior at least two effective factors or equivalent
units. Investigation of the F2 population of a cross, (9)Ex
x (0)Ex, revealed that their heritability of heading days was
approximately 48.4% (VF2>VE). Three offtypes out
of 762 plants of the F2 were produced and they headed much
earlier than T65 and later than Ex-lines. Frequency of offtype
estimated was to be 0.39%. F3 lines of these offtypes showed
a 3 early-type: 1 late-type (T65-like) segregation. The F4
families of these F3 lines were divided into 1 early-lines:
2 segregating-lines: 1 late-lines groups. Seven F5 late fixed
lines were further crossed with T65 and proved, by allelism test, that
these late lines had the ef1 alleles of T65. The Ef1-x locus
may be a complex structure, and the three offtypes may have been heterozygous
for this gene. The progeny plants raised would be recombined from the
heterozygous genotype. |
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