grain_icon Markers Home | Markers Search | Browse Map Sets | SSR Markers Resource | Microarray Probes | Help | Tutorial | FAQs
Find: Type: Taxonomy:
E.g., "OA_BBa0071H05,c0346J09", "rm*", Oat RFLPs like "CDO*", or view help.
expand all sections collapse all sections  Oryza sativa QTL "CQO3"
ID 50707
Name CQO3 (QTL ACCESSION {description)
Synonyms (1) CQO3-COLDTL
Published Symbol {descriptionctb-2
TO TERM {descriptionTO:0000303 (cold tolerance)
Trait Namecold tolerance
Trait SymbolCOLDTL
Type QTL
Species Oryza sativa (Oryza sativa) [ GR_tax:013681 ]
Germplasm
Description The QTL was identified in the NIL derived from 12 recombinants in a segregating
population BT4 derived from a single plant heterozygous for introgression on
Chr4. This plant was derived from a BC2 population of a cross between Syo6 and
Kirara397. Syo6 contains introgressed segments on chr3 and chr4 from BC1F5 lines
derived from the cross Kirara397/Norin-PL8//Kirara397. Norin-PL8 and Kirara397
are cold-tolerant and cold-sensitive varieties respectively. MAPL97 used for
interval mapping and StatView 5.0 was used for analysis of variance between
marker classes. The trait cold tolerance was assayed by using the cool-water
irrigation method. After the seed ripening stage, the cold tolerance was
evaluated on the basis of mean seed fertility. The experiment was carried out
both in the field and green house. The QTL is closely associated with the QTL
for large anther length (QTL accession:CQO4) present in the same region. The
gene OsAOX1a for terminal alternative oxidase (Gramene Protein Accession:
O82807) was mapped in the same region of the QTL. The sequencing of the gene
product and western blot analyses revealed a single nucleotide polymorphism
(SNP) or allelic variation for the gene OsAOX1a among the parents Kirara397 and
Norin-PL8. The Kirara397 allele accries a base subsitution at position 297 in
codon AAG (Lys71). It is replaced by T in codon AAT (Asn71) in Norin-PL8. The
western blot analyses revealed a 32 kDa and 34kDa bands for OsAOX1a in Kirara397
and Norin-PL8 respectively. There is another gene OsAOX1b for terminal
alternative oxidase (Gramene Protein Accession: O82766) which also maps in the
same region as that for OsAOX1a (Gramene curation). The study involving OsAOX1a
also suggests that OsAOX1b neither the protein or its transcripts were detected,
thus suggesting a strong correlation between the QTL and the gene OsAOX1a.
Tags () None
box  Source/Library
toggle section  Details
toggle section  Database Cross-References (2)
box  Sequences (0)
toggle section  Map Positions (2)
toggle section  Associations (13)
toggle section  Associated Ontologies (12)
box  Images (0)