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E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "Identification of two closely linked quantitative trait loci for cold tolerance on chromosome 4 of rice and their association with anther length"
Reference ID 7827
Title Identification of two closely linked quantitative trait loci for cold tolerance on chromosome 4 of rice and their association with anther length
Source Theoretical and applied genetics, 2001, vol. 103(6-7), pp. 862-868
Authors (6)
Abstract Norin-PL8 is a cold-tolerant variety of rice (Oryza sativa L.) that was
developed by introgressing chromosomal segments from a cold-tolerant javanica
variety, Silewah. We previously detected quantitative trait loci (QTLs) for cold
tolerance of Norin-PL8 in the introgressions on chromosomes 3 and 4. We provide
fine mapping of the QTLs on chromosome 4 and the association between the QTLs
and anther length, which has been reported to be a major component of cold
tolerance. Interval mapping using a segregating population derived from an
advanced backcross progeny indicated that a QTL for cold tolerance is probably
located from the center to the proximal end of the introgression. For fine
mapping, we developed a set of near-isogenic lines (NILs) from recombinants in
the segregating population. Comparison of cold tolerance between the NILs
indicated that either the proximal end or the center of the introgression is
necessary for cold tolerance. From these results, we concluded that there are at
least two QTLs for cold tolerance, tentatively designated as Ctb-1 and Ctb-2, in
the introgression on chromosome 4. The map distance between Ctb-1 and Ctb-2 is
estimated to be 4.7-17.2 cM. In order to investigate the mechanism underlying
cold tolerance by the QTLs, we compared anther lengths of the NILs. The results
indicate that both Ctb-1 and Ctb-2 are associated with anther length.

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