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expand all sections collapse all sections  Oryza sativa QTL "CQE12"
ID 50620
Name CQE12 (QTL ACCESSION {description)
Synonyms (1) CQE12-RUBISCOSPROTRO
TO TERM {descriptionTO:0000324 (rubisco to soluble protein ratio)
Trait Namerubisco to soluble protein content
Trait SymbolRUBISCOSPROTRO
Type QTL
Species Oryza sativa (Oryza sativa) [ GR_tax:013681 ]
Germplasm
Description Trait assayed for Rubisco to total soluble protein content. Soluble protein
concentration in the supernatant was determined by the method of Bradford with
bovine serum albumin as a standard. The protein extract was prepared from leaves
by grinding them in liquid nitrogen to a fine powder and suspended in a buffer
containing 100 mM NaH2PO4 (pH 7.0), 1 mM phenylmethylsulfonyl fluoride, 1% (w/v)
insoluble polyvinyl polypyrrolidone, and 1% (v/v) beta-mercaptoethanol, and then
centrifuged at 9,800 g for 5 min. For determining Rubisco content soluble
proteins (2 µg) were subjected to SDS-PAGE using 12.5% (w/v) gels containing
0.1% (w/v) SDS, and the gels were stained with Coomassie brilliant blue. The
intensity of the band corresponding to Rubisco was measured with an image
analyzer (Umax Technologies, Calif.). The means of ten replications per line
were used in the data analysis. Phenotypic effect contributed by Nipponbare
allele. Chromosomal locations of putative QTLs were determined by single-factor
analysis (one-way ANOVA) with the general linear model procedure of QGENE. Two-
way ANOVAs for epistatic interaction were also carried out with QGENE at Pd0.01
threshold. The tests were done only with marker loci linked to QTLs with a single-
factor P of d0.01 to reduce the likelihood of committing a type-I error.
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