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expand all sections collapse all sections  Oryza sativa QTL "CQF3"
ID 50586
Name CQF3 (QTL ACCESSION {description)
Synonyms (1) CQF3-SPROTCN
TO TERM {descriptionTO:0000325 (soluble protein content)
Trait Namesoluble protein content
Trait SymbolSPROTCN
Type QTL
Species Oryza sativa (Oryza sativa) [ GR_tax:013681 ]
Germplasm
Description The soluble protein concentration in the supernatant was determined by the
method of Bradford with bovine serum albumin as a standard. The protein extract
was prepared from leaves by grinding suspended in a buffer containing 100 mM
NaH2PO4 (pH 7.0), 1 mM phenylmethylsulfonyl fluoride, 1% (w/v) insoluble
polyvinyl polypyrrolidone, and 1% (v/v) beta-mercaptoethanol, and then
centrifuged at 9,800 g for 5 min. The analysis was done on contents derived from
leaf tissue collected 5 days after heading. The means of ten replications per
line were used in the data analysis. Phenotypic effect contributed by Nipponbare
allele. Chromosomal locations of putative QTLs were determined by single-factor
analysis (one-way ANOVA) with the general linear model procedure of QGENE. A
probability level of 0.01 was used as the threshold for detecting significant
differences at P < 0.001 in the mean values of the two genotypic classes,
homozygous for Nipponbare and Kasalath alleles. In the back crossed inbred lines
(BILs) there was a high correlation among the soluble protein content and
Rubisco content at 5 days (r=0.554; P<0.01) after heading.
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