QTL Accession ID | CQF3 |
---|---|
Species | Oryza sativa (Rice) [ GR_tax:013681 ] |
Trait Symbol | SPROTCN |
Trait Name | soluble protein content |
Published Symbol | |
Trait Synonym(s) | Soluble/Total protein |
Trait Category | Biochemical |
Chromosome | 1 |
Comments | The soluble protein concentration in the supernatant was determined by the method of Bradford with bovine serum albumin as a standard. The protein extract was prepared from leaves by grinding suspended in a buffer containing 100 mM NaH2PO4 (pH 7.0), 1 mM phenylmethylsulfonyl fluoride, 1% (w/v) insoluble polyvinyl polypyrrolidone, and 1% (v/v) beta-mercaptoethanol, and then centrifuged at 9,800 g for 5 min. The analysis was done on contents derived from leaf tissue collected 5 days after heading. The means of ten replications per line were used in the data analysis. Phenotypic effect contributed by Nipponbare allele. Chromosomal locations of putative QTLs were determined by single-factor analysis (one-way ANOVA) with the general linear model procedure of QGENE. A probability level of 0.01 was used as the threshold for detecting significant differences at P < 0.001 in the mean values of the two genotypic classes, homozygous for Nipponbare and Kasalath alleles. In the back crossed inbred lines (BILs) there was a high correlation among the soluble protein content and Rubisco content at 5 days (r=0.554; P<0.01) after heading. |
Map Type | Map Set | Name | Map | Position | Ext. Links | |||
---|---|---|---|---|---|---|---|---|
Sequence | Gramene Annotated Nipponbare Sequence 2009 | BNL6.32 | Chr. 1 | 42,412,421-43,236,916 bp | View Comparative Map | |||
|
Map Type | Map Set | Name | Map | Position | Ext. Links | |||
---|---|---|---|---|---|---|---|---|
QTL | JRGP Nip/Kas F2 QTL 2000 | BNL6.32 | 1 | 176.3-181.8 cM | View Comparative Map | |||
|
Name | Chromosome | Start | Stop |
---|---|---|---|
CQF3 | 1 | 176.3 | 181.8 |
Direction | Name | Type | Species | Assoc. Type |
---|---|---|---|---|
To | C112 | RFLP | Oryza sativa | qtl_marker |
To | C12249S | RFLP | Oryza sativa | qtl_marker |
To | G5005 | RFLP | Oryza sativa | qtl_marker |
To | R117 | RFLP | Oryza sativa | qtl_marker |
To | Y2320L | RFLP | Oryza sativa | qtl_marker |
To | E61984S | RFLP | Oryza sativa | qtl_marker |
To | E60152S | RFLP | Oryza sativa | qtl_marker |
To | S13931 | RFLP | Oryza sativa | qtl_marker |
To | R2727 | RFLP | Oryza sativa | qtl_marker |
To | S21582S | RFLP | Oryza sativa | qtl_marker |
To | Y2320R | RFLP | Oryza sativa | qtl_marker |
Term Type | Term | Evidence |
---|---|---|
Trait Ontology | (TO:0000325) | Ishimaru-K et al., 2001 (IAGP) |
Plant Structure | (PO:0020103) | Ishimaru-K et al., 2001 (SM) |
(PO:0009010) | Gramene curators, 2007 (IC) | |
Plant Growth and Development Stage | (PO:0007038) | Ishimaru-K et al., 2001 (SM) |
Growth Stage | (GRO:0007045) | Ishimaru-K et al., 2001 (SM) |
Environment | (EO:0007403) | Gramene curators, 2007 (IC) |
Species Ontology | (GR_tax:013681) | Ishimaru-K et al., 2001 (SM) |
Chr | Start | Stop | Exp | Num. SNP Positions | |
---|---|---|---|---|---|
chr1 | 42,412,421 | 43,236,916 | ~160K SNPs x 20 accessions (McNally 2... | 950 | Search for SNPs |
chr1 | 42,412,421 | 43,236,916 | '1536 chip' - 1311 SNPs x 395 rice ac... | 5 | Search for SNPs |
chr1 | 42,412,421 | 43,236,916 | Huang X, et al, Nat Genetics 2010 Nov... | 2,338 | Search for SNPs |
chr1 | 42,412,421 | 43,236,916 | RiceDiversity 44K SNP assay, 34 trait... | 2,338 | Search for SNPs |
chr1 | 42,412,421 | 43,236,916 | RiceDiversity 44K SNP assay, 34 trait... | 568 | Search for SNPs |