Reference "Mapping of QTLs associated with cold tolerance during the vegetative stage in rice"

Reference ID

8161

Title

Mapping of QTLs associated with cold tolerance during the vegetative stage in rice

Source

Journal of experimental botany, 2003, vol. 54, pp. 2579-2585

Authors (2)

Andaya-V-C

Mackill-D-J

Abstract

Low-temperature stress is an important factor affecting the growth and
development of rice (Oryza sativa L.) in temperate and high-elevation areas.
Cold stress may cause various seedling injuries, delayed heading and yield
reduction due to spikelet sterility. In this study, 181 microsatellite marker
loci were used to identify quantitative trait loci (QTLs) associated with cold
tolerance at the vegetative stage in 191 recombinant inbred lines (RILs) derived
from a cross of a cold-tolerant temperate japonica cultivar (M-202) with a cold-
sensitive indica cultivar (IR50). Different temperature regimes were applied in
growth chambers on 191 RILs. The temperature regimes imposed in the growth
chamber simulated cold-stress injuries at the seedling and late vegetative
stages. In this study a major QTL was identified on chromosome 12, designated as
qCTS12a, that was closely associated with cold-induced necrosis and wilting
tolerance, and accounted for 41% of the phenotypic variation. A number of QTLs
with smaller effects were also detected on eight rice chromosomes.