Reference "Identification of aluminium-regulated genes by cDNA-AFLP in rice (Oryza sativa L.): aluminium-regulated genes for the metabolism of cell wall components"

Reference ID

8095

Title

Identification of aluminium-regulated genes by cDNA-AFLP in rice (Oryza sativa L.): aluminium-regulated genes for the metabolism of cell wall components

Source

Journal of experimental botany, 2004, vol. 55, pp. 137-143

Authors (7)

Mao-C	Yi-K
Yang-L	Zheng-B
Wu-Y	Liu-F
Wu-P

Abstract

Aluminium (Al) toxicity is the major factor limiting crop productivity in acid
soils. To investigate the molecular mechanisms of Al toxicity and Al tolerance
of rice, cDNA-amplified fragment length polymorphism (cDNA-AFLP) was used for
identifying Al-regulated genes in roots of an Al-tolerant tropical upland rice,
Azucena, and an Al-sensitive lowland rice, IR1552. Nineteen function-known genes
were found among 34 transcript-derived fragments (TDFs) regulated by Al stress.
The results indicate that Al stress could induce the biosynthesis of lignin and
other cell wall components in roots. Temporal expression patterns of 14 genes
were identified between the two varieties. In silico mapping was performed for
all the 33 unique genes. Two genes for a function-unknown protein and for a ubiquitin-
like protein, respectively, were mapped on the interval with the common QTL
(quantitative trait loci) for Al tolerance in rice on chromosome 1.