Reference "Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice"

Reference ID

7869

Title

Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice

Source

Theoretical and applied genetics, 2000, vol. 100, pp. 1121-1128

Authors (5)

Hittalmani-S	Parco-A
Mew-T-V	Zeigler-R-S
Huang-N

Abstract

Three major genes (Pi1, Piz-5 and Pita) for blast resistance on chromosomes 11,
6 and 12, respectively, were fine-mapped and closely linked RFLP markers
identified. New markers for Pi1 and Pita were found that were flanking the
genes. The three genes were pyramided using RFLP markers. A PCR-based SAP
(sequence amplified polymorphism) marker was used to identify Piz-5 in the
segregating population. The plants carrying the two- and three-gene
combinations that were tested for resistance to leaf blast in the Philippines
and India indicated that combinations including Piz-5 have enhanced resistance
than when it is present alone. The genes from the pyramided lines are at
present being deployed into agronomically superior rice varieties by marker-
aided selection (MAS).