Reference "Functional analysis in yeast of cDNA coding for the mitochondrial Rieske iron-sulfur protein of higher plants"

Reference ID

7385

Title

Functional analysis in yeast of cDNA coding for the mitochondrial Rieske iron-sulfur protein of higher plants

Source

Proceedings of the National Academy of Sciences of the United States of America, 1991, vol. 88, pp. 10716-10720

Authors (3)

Huang-J-T	Struck-F
Matzinger-D-F

Abstract

cDNA clones coding for the nuclear-encoded mitochondrial Rieske iron-sulfur
protein (RISP) have been isolated from maize and tobacco. Complementation
analysis of hybrid proteins consisting of different domains of plant and yeast
RISPs showed that the carboxyl two-thirds of the plant protein is functionally
equivalent to that of the yeast protein. The amino terminus of the RISP,
however, seems to be species specific because this region is not interchangeable
between plant and yeast proteins. Complementation analysis of hybrid proteins
also identified a structurally conserved domain probably essential for the
function of bc1 complex RISPs. A specific domain from the plant RISP was found
to cause temperature-sensitive respiratory growth in yeast. We have demonstrated
that yeast can serve as a model system to study the structural and functional
relationships of plant gene products that are enzymatic components of the
mitochondrial respiratory chain.