Reference "A genetic and physical map of the region containing PLASTOCHRON1, a heterochronic gene, in rice (Oryza sativa L.)"

Reference ID

7010

Title

A genetic and physical map of the region containing PLASTOCHRON1, a heterochronic gene, in rice (Oryza sativa L.)

Source

Theoretical and applied genetics, 2002, vol. 105, pp. 654-659

Authors (5)

Ahn-B-O	Miyoshi-K
Itoh-J-I	Nagato-Y
Kurata-N

Abstract

The rice heterochronic gene plastochron1, pla1, shows shorter plastochron and
ectopic expression of the vegetative program during the rice reproductive phase
resulting in aberrant panicle formation. A genetic and physical map was
constructed to isolate the causal gene for the pla1 syndrome. Small-scale
mapping was carried out to determine the approximate map position of the pla1
locus, and then a high-resolution genetic map was made for pla1-1, one of the
pla1 alleles, using an F2 population comprising 578 pla1-1 homozygous plants. In
a high-resolution genetic map, the pla1-1 locus was found to map between RFLP
markers C961 and R1738A on chromosome 10, within a 3.6-cM genetic distance. A
physical map encompassing the pla1-1 locus was constructed by overlapping
Bacterial Artificial Chromosome (BAC) clones through chromosome walking. PCR-
based RFLP markers from BAC-end clones were developed and mapped relative to the
pla1 locus. Physical map construction using BAC clones indicated that a BAC
clone, B44A10 (167-kb), contained the pla1 locus within 74-kb corresponding to a
0.52-cM genetic distance. Gene prediction of 74-kb region carrying the pla1
locus suggested several candidate genes for the pla1 gene. Identification of a
candidate gene for pla1 will be made by sequence analysis of allele variation
and cDNA screening.