Reference "A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein"

Reference ID

6995

Title

A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein

Source

Proceedings of the National Academy of Sciences of the United States of America, 2002, vol. 99, pp. 7530-7535

Authors (5)

Yamanouchi-U	Yano-M
Lin-H	Ashikari-M
Yamada-K

Abstract

A rice spotted leaf (lesion-mimic) gene, Spl7, was identified by map-based
cloning. High-resolution mapping with cleaved amplified polymorphic sequence
markers enabled us to define a genomic region of 3 kb as a candidate for Spl7.
We found one ORF that showed high similarity to a heat stress transcription
factor (HSF). Transgenic analysis verified the function of the candidate gene
for Spl7: leaf spot development was suppressed in spl7 mutants with a wild-type
Spl7 transgene. Thus, we conclude that Spl7 encodes the HSF protein. The
transcript of spl7 was observed in mutant plants. The levels of mRNAs (Spl7 in
wild type and spl7 in mutant) increased under heat stress. Sequence analysis
revealed only one base substitution in the HSF DNA-binding domain of the mutant
allele, causing a change from tryptophan to cysteine.