Reference "Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis"

Reference ID

6565

Title

Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis

Source

DNA research : an international journal for rapid publication of reports on genes and genomes, 2002, vol. 9, pp. 11-17

Authors (10)

Monna-L	Kitazawa-N
Yoshino-R	Suzuki-J
Masuda-H	Maehara-Y
Tanji-M	Sato-M
Nasu-S	Minobe-Y

Abstract

A rice semidwarfing gene, sd-1, known as the "green revolution gene," was
isolated by positional cloning and revealed to encode gibberellin 20-oxidase,
the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477
segregants using several PCR-based marker technologies, including cleaved
amplified polymorphic sequence, derived-CAPS, and single nucleotide
polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice
cultivars have an identical sequence in this region, consisting of 3 exons
(558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type
sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the
expressed sequence), from the middle of exon 1 to upstream of exon 2, including
a 105-bp intron, resulting in a frame-shift that produces a termination codon
after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-
bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe
[TTC]). Expression analysis suggests the existence of at least one more locus
of gibberellin 20-oxidase which may prevent severe dwarfism from developing in
sd-1 mutants.