Reference "Early flowering and reduced apical dominance result from ectopic expression of a rice MADS box gene"

Reference ID

591

Title

Early flowering and reduced apical dominance result from ectopic expression of a rice MADS box gene

Source

Plant molecular biology, 1994, vol. 26, pp. 657-665

Authors (5)

Chung-Y-Y	Kim-S-R
Finkel-D	Yanofsky-M-F
An-G

Abstract

Recent studies with dicot plants reveal that floral organ development is
controlled by a group of regulatory factors containing the MADS domain. In this
study, we have isolated and characterized a cDNA clone from rice, OsMADS1, which
encodes a MADS-domain-containing protein. The OsMADS1 amino acid sequence shows
56.2% identity to AGL2 and 44.4% identity to AP1. The MADS box region was the
most homologous to other MADS-domain-containing proteins. Northern blot analysis
indicated that the rice MADS gene was preferentially expressed in floral organs.
In situ localization studies showed that the transcript was uniformly present in
young flower primordia and later became localized in palea, lemma, and ovary.
Ectopic expression of OsMAD1 with the CaMV 35S promoter in transgenic tobacco
plants dramatically alters development, resulting in short, bushy, early-
flowering plants with reduced apical dominance. These results suggest that the
OsMADS1 gene is involved in flower induction and that it may be used for genetic
manipulation of certain plant species.