Reference "Chromosomal locations of ten isozyme in rice (Oryza sativa L.) through trisomic analysis"

Reference ID

4973

Title

Chromosomal locations of ten isozyme in rice (Oryza sativa L.) through trisomic analysis

Source

Biochemical genetics, 1988, vol. 26, pp. 303-320

Authors (3)

Wu-K-S	Glaszmann-J-C
Khush-G-S

Abstract

Chromosomal locations of 10 isozyme loci in rice (Oryza sativa L.) were
determined through trisomic analysis. All 10 genes produced altered allozyme
banding patterns in specific F1 trisomics. This served as the primary source of
evidence for chromosome locations of Est-5, Icd-1, Acp-1, and Pgd-1. The
locations of Amp-1, Amp-2, Amp-4, Pox-5, Got-1, and Cat-1 were further confirmed
from segregation data in BC1 generations, as the ratios deviated significantly
from 1:1 in the critical trisomics but agreed with the expected trisomic ratios.
Triallelic heterozygotes were recovered for Amp-1 and Amp-2. On the basis of
these data Got-1, Est-5, and Icd-1 were located to chromosome 1, Amp-1 to
chromosome 2, Cat-1 and Pox-5 to chromosome 3, Acp-1 to chromosome 6, Amp-2 and
Amp-4 to chromosome 8, and Pgd-1 to chromosome 11. Because Acp-2 and Pox-2 are
known to be linked with Acp-1, they must also be on chromosome 6. The gene order
and recombination values between isozyme loci on chromosomes 3, 6, 8, and 11 are
presented.