Reference "Mapping of rice Rf gene by bulked line analysis"

Reference ID

4450

Title

Mapping of rice Rf gene by bulked line analysis

Source

DNA research : an international journal for rapid publication of reports on genes and genomes, 1998, vol. 5, pp. 15-18

Authors (4)

Tan-X-L	Vanavichit-A
Amornsilpa-S	Trangoonrung-S

Abstract

A method, bulked line analysis (BLA), was developed for identification of the
RFLP markers associated with a target gene. Instead of segregating progenies,
conventional lines sharing the same trait were bulked by the BLA method. This
method is an alternative approach to the identification of DNA markers linked
with a target gene. A major advantage of this method is time-saving for genetic
stock development. The advantage is very significant for organisms having a
long generation period. This method has been tested by using fertility
restoration of rice cytoplasmic male sterility of wild abortive type as a
target trait. A fertility-restoring gene was successfully identified by linkage
with RFLP markers. This gene was mapped in the middle of the long arm of
chromosome 10 of the rice genome.