Reference "Rice class III chitinase homologues isolated by random cloning of rice cDNAs"

Reference ID

3030

Title

Rice class III chitinase homologues isolated by random cloning of rice cDNAs

Source

DNA research : an international journal for rapid publication of reports on genes and genomes, 1997, vol. 4, pp. 379-385

Authors (8)

Nagasaki-H	Yamamoto-K
Shomura-A	Koga-Ban-Y
Takasuga-A	Yano-M
Minobe-Y	Sasaki-T

Abstract

We obtained seven kinds of cDNA clones putatively identified as encoding class
III chitinases from cDNA libraries constructed from dichlorophenoxyacetic acid
(2,4-D)- and benzyl adenine (BA)- treated rice callus. Putative amino acid
sequences encoded in these cDNA clones were compared with those of known
chitinases of other plants. Two clones coded for homologues that show high
similarity to class III chitinases. These clones contained the common glutamic
acid at the active site and were classified as true homologues. The other five
clones, however, showed relatively low similarity to class III chitinases and
their active sites contained aspartic acid instead of glutamic acid. These
clones may correspond to relatives of a super family of class III chitinases.
The location of the genes coding for these homologues on the rice genome has
been determined by genetic linkage analysis with restriction fragment length
polymorphism.