Reference "A 300 kilobase interval genetic map of rice including 883 expressed sequences"

Reference ID

2293

Title

A 300 kilobase interval genetic map of rice including 883 expressed sequences

Source

Nature genetics, 1994, vol. 8, pp. 365-372

Authors (28)

Kurata-N	Nagamura-Y	Yamamoto-K	Harushima-Y	Sue-N	Wu-J
Antonio-B-A	Shomura-A	Shimizu-T	Lin-S-Y	Inoue-T	Fukuda-A
Shimano-T	Kuboki-Y	Toyama-T	Miyamoto-Y	Kirihara-T	Hayasaka-K
Miyao-A	Monna-L	Zhong-H-S	Tamura-Y	Wang-Z-X	Momma-T
Umehara-Y	Yano-M	Sasaki-T	Minobe-Y

Abstract

We have constructed a high resolution rice genetic map containing 1,383 DNA
markers at an average interval of 300 kilobases (kb). The markers, distributed
along 1,575 cM on 12 linkage groups, comprise 883 cDNAs, 265 genomic DNAs, 147
randomly amplified polymorphic DNAs (RAPD) and 88 other DNAs. cDNAs were derived
from rice root and callus, analysed by single-run sequencing and searched for
similarities with known proteins. Nearly 260 rice genes are newly identified and
mapped, and genomic DNA and cloned RAPD fragments were also sequenced to
generate STSs. Our map is the first significant gene expression map in plants.
It is also the densest genetic map available in plants and the first to be
backed up comprehensively by clone sequence data.