Reference "Gibberellin biosynthesis: metabolic evidence for three steps in the early 13-hydroxylation pathway of rice"

Reference ID

2148

Title

Gibberellin biosynthesis: metabolic evidence for three steps in the early 13-hydroxylation pathway of rice

Source

Phytochemistry, 2000, vol. 55, pp. 317-321

Authors (6)

Kobayashi-M	MacMillan-J
Phinney-B	Gaskin-P
Spray-C-R	Hedden-P

Abstract

[14C4]GA53, [14C4]GA44, and [2H2/14C4]GA19 were injected separately into
seedlings of rice (Oryza sativa) using a dwarf mutant (d35) that has low levels
of endogenous gibberellins (GAs). After 8 h incubation, the shoots were
extracted and the labeled metabolites were identified by full-scan gas
chromatography mass spectrometry (GC-MS) and Kovats retention indices (KRIs).
Our results document the metabolic sequence, GA53-->GA44-->GA19-->GA20 and the
presence of endogenous GA53, GA44, GA19, GA20 and GA1. Previous metabolic
studies have shown the presence of the step, GA20-->GA1 in rice. Taken together,
the data establish in vegetative shoots of rice the presence of the early 13-
hydroxylation pathway, a pathway that originates from GA12 and leads to
bioactive GA1.