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E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "RNA maturation of the rice SPK gene may involve trans-splicing"
Reference ID 1982
Title RNA maturation of the rice SPK gene may involve trans-splicing
Source The Plant journal : for cell and molecular biology, 1999, vol. 18, pp. 625-632
Authors (6)
Abstract A gene encoding a calcium-dependent seed-specific protein kinase (SPK) is
abundantly expressed in developing rice seeds (Kawasaki, T et al. Gene (1993)
129, 183-189). Rice genomic clones encoding SPK were isolated using the entire
cDNA fragment as a probe. Physical mapping of these genomic clones indicated
that the genomic region corresponding to the entire cDNA was divided into two
different regions, SPK-A and SPK- B, located on different rice chromosomes. The
results of RACE-PCR analyses showed that the respective transcripts from SPK-A
and SPK-B contained additional sequences which were not found in the SPK cDNA,
and that these sequences were removed like introns during maturation of the SPK
mRNA. These results suggest that two different RNAs were independently
transcribed from SPK-A and SPK-B and joined, possibly by trans-splicing.

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