Reference "leafy hull sterile1 is a homeotic mutation in a rice MADS box gene affecting rice flower development"

Reference ID

1772

Title

leafy hull sterile1 is a homeotic mutation in a rice MADS box gene affecting rice flower development

Source

The Plant cell, 2000, vol. 12, pp. 871-884

Authors (11)

Jeon-J-S	Jang-S	Lee-S	Nam-J
Kim-C	Lee-S-H	Chung-Y-Y	Kim-S-R
Lee-Y-H	Cho-Y-G	An-G

Abstract

Rice contains several MADS box genes. It has been demonstrated previously that
one of these genes, OsMADS1 (for Oryza sativa MADS box gene1), is expressed
preferentially in flowers and causes early flowering when ectopically expressed
in tobacco plants. In this study, we demonstrated that ectopic expression of
OsMADS1 in rice also results in early flowering. To further investigate the role
of OsMADS1 during rice flower development, we generated transgenic rice plants
expressing altered OsMADS1 genes that contain missense mutations in the MADS
domain. There was no visible alteration in the transgenic plants during the
vegetative stage. However, transgenic panicles typically exhibited phenotypic
alterations, including spikelets consisting of elongated leafy paleae and lemmas
that exhibit a feature of open hull, two pairs of leafy palea-like and lemma-
like lodicules, a decrease in stamen number, and an increase in the number of
carpels. In addition, some spikelets generated an additional floret from the
same rachilla. These characteristics are very similar to those of leafy hull
sterile1 (lhs1). The map position of OsMADS1 is closely linked to that of lhs1
on chromosome 3. Examination of lhs1 revealed that it contains two missense
mutations in the OsMADS1 MADS domain. A genetic complementation experiment
showed that the 11.9-kb genomic DNA fragment containing the wild-type OsMADS1
gene rescued the mutant phenotypes. In addition, ectopic expression of the
OsMADS1 gene isolated from the lhs1 line resulted in lhs1-conferred phenotypes.
These lines of evidence demonstrate that OsMADS1 is the lhs1 gene.