Reference "Diterpene Cyclases Responsible for the Biosynthesis of Phytoalexins, Momilactones A, B, and Oryzalexins A

Reference ID

11691

Title

Diterpene Cyclases Responsible for the Biosynthesis of Phytoalexins, Momilactones A, B, and Oryzalexins A–F in Rice

Source

Bioscience, biotechnology, and biochemistry, 2004, vol. 68, pp. 2001-2006

Authors (12)

Otomo-K	Kanno-Y	Motegi-A	Kenmoku-H
Yamane-H	Mitsuhashi-W	Oikawa-H	Toshima-H
Itoh-H	Matsuoka-M	Sassa-T	Toyomasu-T

Abstract

Rice (Oryza sativa L.) produces diterpene phytoalexins, such as momilactones,
oryzalexins, and phytocassanes. Using rice genome information and in vitro assay
with recombinant enzymes, we identified genes (OsKS4 and OsKS10) encoding the
type-A diterpene cyclases 9β-pimara-7,15-diene synthase and ent-
sandaracopimaradiene synthase which are involved in the biosynthesis of
momilactones A, B and oryzalexins A–F respectively. Transcript levels of these
two genes increased remarkably after ultraviolet (UV) treatment, which is
consistent with elevated production of phytoalexins by UV. These two genes might
prove powerful tools for understanding plant defense mechanisms in rice.