Reference "Overexpression of the OsZIP4 zinc transporter confers disarrangement of zinc distribution in rice plants"

Reference ID

11643

Title

Overexpression of the OsZIP4 zinc transporter confers disarrangement of zinc distribution in rice plants

Source

Journal of experimental botany, 2007, vol. , pp. -

Authors (8)

Ishimaru-Y	Masuda-H
Suzuki-M	Bashir-K
Takahashi-M	Nakanishi-H
Mori-S	Nishizawa-N-K

Abstract

Zinc (Zn), an essential nutrient in cells, plays a vital role in controlling
cellular processes such as growth, development, and differentiation. Although
the mechanisms of Zn translocation in rice plants (Oryza sativa) are not fully
understood, it has recently received increased interest. OsZIP4 is a Zn
transporter that localizes to apical cells. Transgenic rice plants
overexpressing the OsZIP4 gene under the control of the cauliflower mosaic virus
(CaMV) 35S promoter were produced. The Zn concentration in roots of 35S-OsZIP4
transgenic plants was 10 times higher than in those of vector controls, but it
was five times lower in shoots. The Zn concentration in seeds of 35S-OsZIP4
plants was four times lower compared with vector controls. Northern blot
analysis and quantitative real-time reverse transcription-PCR revealed
transcripts of OsZIP4 expression driven by the CaMV 35S promoter in roots and
shoots of 35S-OsZIP4 plants, but levels of endogenous OsZIP4 transcripts were
low in roots and high in shoots compared with vector controls. Microarray
analysis revealed that the genes expressed in shoots of 35S-OsZIP4 plants
coincided with those induced in shoots of Zn-deficient plants. These results
indicate that constitutive expression of OsZIP4 changes the Zn distribution
within rice plants, and that OsZIP4 is a critical Zn transporter that must be
strictly regulated.