Reference "Suppression of RICE TELOMERE BINDING PROTEIN1 Results in Severe and Gradual Developmental Defects Accompanied by Genome Instability in Rice"

Reference ID

11624

Title

Suppression of RICE TELOMERE BINDING PROTEIN1 Results in Severe and Gradual Developmental Defects Accompanied by Genome Instability in Rice

Source

The Plant cell, 2007, vol. , pp. -

Authors (8)

Hong-J-P	Byun-M-Y
Koo-D-H	An-K
Bang-J-W	Chung-I-K
An-G	Kim-W-T

Abstract

Although several potential telomere binding proteins have been identified in
higher plants, their in vivo functions are still unknown at the plant level.
Both knockout and antisense mutants of RICE TELOMERE BINDING PROTEIN1 (RTBP1)
exhibited markedly longer telomeres relative to those of the wild type,
indicating that the amount of functional RTBP1 is inversely correlated with
telomere length. rtbp1 plants displayed progressive and severe developmental
abnormalities in both germination and postgermination growth of vegetative
organs over four generations (G1 to G4). Reproductive organ formation, including
panicles, stamens, and spikelets, was also gradually and severely impaired in G1
to G4 mutants. Up to 11.4, 17.2, and 26.7% of anaphases in G2, G3, and G4 mutant
pollen mother cells, respectively, exhibited one or more chromosomal fusions,
and this progressively increasing aberrant morphology was correlated with an
increased frequency of anaphase bridges containing telomeric repeat DNA.
Furthermore, 35S:anti-RTBP1 plants expressing lower levels of RTBP1 mRNA
exhibited developmental phenotypes intermediate between the wild type and
mutants in all aspects examined, including telomere length, vegetative and
reproductive growth, and degree of genomic anomaly. These results suggest that
RTBP1 plays dual roles in rice (Oryza sativa), as both a negative regulator of
telomere length and one of positive and functional components for proper
architecture of telomeres.