Reference ID | 11447 | ||||||||
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Title | Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare) | ||||||||
Source | Biotechnol Lett, 2007, vol. , pp. - | ||||||||
Authors (7) |
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Abstract | The putative raffinose synthase gene from rice was cloned and expressed in Escherichia coli. The enzyme displayed an optimum activity at 45 degrees C and pH 7.0, and a sulfhydryl group was required for its activity. The enzyme was specific for galactinol and p-nitrophenyl-alpha-D: -galactoside as galactosyl donors, and sucrose, lactose, 4-beta-galactobiose, N-acetyl-D: -lactosamine, trehalose and lacto-N-biose were recognized as galactosyl acceptors. |
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