Reference "Morphogenesis and molecular basis on naked seed rice, a novel homeotic mutation of OsMADS1 regulating transcript level of AP3 homologue in rice"

Reference ID

11424

Title

Morphogenesis and molecular basis on naked seed rice, a novel homeotic mutation of OsMADS1 regulating transcript level of AP3 homologue in rice

Source

Planta, 2006, vol. 223, pp. 882-890

Authors (6)

Chen-Z-X	Wu-J-G
Ding-W-N	Chen-H-M
Wu-P	Shi-C-H

Abstract

The floral organs are formed from floral meristem with a regular initiation
pattern in angiosperm species. Flowers of naked seed rice (nsr) were
characterized by the overdeveloped lemma and palea, the transformation of
lodicules to palea-/lemma-like organs, the decreased number of stamens and
occasionally extra pistils. Some nsr spikelets contained additional floral
organs of four whorls and/or abnormal internal florets. The floral primordium of
nsr spikelet is differentiated under an irregular pattern and an incomplete
determination. And molecular analysis indicated that nsr was a novel homeotic
mutation in OsMADS1, suggesting that OsMADS1 played a distinct role in
regulating the differentiation pattern of floral primordium and in conferring
the determination of flower meristem. The gain-of-function of OsMADS1 transgenic
lines presented the transformation of outer glumes to lemma-/palea-like organs
and no changes in length of lemma and palea, but loss-of-function of OsMADS1
transgenic lines displayed the overdeveloped lemma and palea. Both findings
revealed that OsMADS1 played a role in specifying lemma and palea and acted as a
repressor of overdevelopment of lemma and palea. Moreover, it was indicated that
OsMADS1 upregulated the transcript level of AP3 homologue OsMADS16, using real-
time PCR analysis on gain- and loss-of-function of OsMADS1 transgenic lines.