Reference "OsFY, a Homolog of AtFY, Encodes a Protein that Can Interact with OsFCA-gamma in Rice (Oryza sativa L.)"

Reference ID

11174

Title

OsFY, a Homolog of AtFY, Encodes a Protein that Can Interact with OsFCA-gamma in Rice (Oryza sativa L.)

Source

Acta Biochim Biophys Sin (Shanghai), 2006, vol. 38, pp. 492-499

Authors (3)

Lu-Q	Xu-Z-K
Song-R-T

Abstract

FCA and FY are flowering time related genes involved in the autonomous
flowering pathway in Arabidopsis. FCA interacts with FY to regulate the
alternative processing of FCA pre-mRNA. The FCA/FY interaction is also required
for the regulation of FLC expression, a major floral repressor in Arabidopsis.
However, it is not clear if the regulation of this autonomous flowering pathway
is also present in monocot plants, such as rice. Recently, alternative RNA
processing of OsFCA was observed in rice, which strongly suggested the
existence of an autonomous flowering pathway in rice. In this work, we cloned
the cDNA of the autonomous flowering pathway gene OsFY from rice. The predicted
OsFY protein contained a conserved 7 WD-repeat region and at least two Pro-Pro-Leu-
Pro motifs compared to Arabidopsis FY. The protein-protein interaction between
OsFY and OsFCA-gamma, the key feature of their gene function, was also
demonstrated using the yeast two-hybrid system. The GenBank database search
provided evidence of expression for other autonomous pathway gene homologs in
rice. These results indicate that the autonomous flowering pathway is present
in monocots, and the regulation through FY and FCA interaction is conserved
between monocots and dicots.