Reference "PAIR2 is essential for homologous chromosome synapsis in rice meiosis I"

Reference ID

11023

Title

PAIR2 is essential for homologous chromosome synapsis in rice meiosis I

Source

Journal of cell science, 2006, vol. 119, pp. 217-225

Authors (5)

Nonomura-K	Nakano-M
Eiguchi-M	Suzuki-T
Kurata-N

Abstract

The PAIR2 gene is required for homologous chromosome synapsis at meiosis I in
rice (Oryza sativa L.) and encodes a HORMA-domain protein that is homologous to
Saccharomyces cerevisiae HOP1 and Arabidopsis ASY1. Immunocytological and
electron microscopic analyses indicate that PAIR2 proteins associate with axial
elements (AEs) at leptotene and zygotene, and is removed from the AEs of arm
regions when homologous chromosomes have been synapsed. Immunocytology against a
centromeric histone H3 variant revealed that PAIR2 remains at centromeres until
diakinesis, by which time the homologous centromeres had already been synapsed.
However, neither precocious segregation of sister chromatids nor kinetochore
dysfunction is observed, and AEs are normally assembled in the mutant. In the
pair2-null mutant, homologous chromosome synapsis is completely eliminated. This
study provides the first description of AE-associated protein in monocot plants
and indicates that PAIR2 plays an essential role in promoting homologous
chromosome synapsis. However, PAIR2 does not play a role in AE formation, sister
chromatid cohesion at centromeres or kinetochore assembly in meiosis I of rice.