Title | Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson |
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Design | Phenotypic evaluation was done in a greenhouse for 353 BC2F1 families and in three field trials for 258 BC2F2 families. Genotyping (104 SSRs and 49 RFLPs) was done on the 353 BC2F2 families |
Polymorphism Type (2) | SSR, RFLP |
Allele Scoring Protocol (2) | Geneotype value 1= Homozygous Jefferson; 3 = Heterozygous Jefferson/O. rufipogon., |
PI(s) | McCouch-S-R |
Comments | Seventy-six QTLs were identified using interval mapping and composite interval mapping. A putative QTL was reported if detected in at least one environment at an experiment-wise significance threshold of P < 0.01. Reference: Thomson et al., 2003. |
Marker Name | |
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CDO105 | Show Allele Data |
CDO1338 | Show Allele Data |
CDO36 | Show Allele Data |
CDO544 | Show Allele Data |
CDO590 | Show Allele Data |
CDO718 | Show Allele Data |
CDO98 | Show Allele Data |
RG1109 | Show Allele Data |
RG118 | Show Allele Data |
RG1356 | Show Allele Data |
RG30 | Show Allele Data |
RG331 | Show Allele Data |
RG351 | Show Allele Data |
RG358 | Show Allele Data |
RG532 | Show Allele Data |
RG811 | Show Allele Data |
RG869 | Show Allele Data |
RG901 | Show Allele Data |
RM10 | Show Allele Data |
RM104 | Show Allele Data |
RM106 | Show Allele Data |
RM119 | Show Allele Data |
RM124 | Show Allele Data |
RM125 | Show Allele Data |
RM126 | Show Allele Data |
Trait Name | Trait Ontology Accession | Phenotype Measurement |
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100-grain weight | TO:0000269 | Grain weight was measured, in grams, as the average weight of 100 kernels. |
Awn length | TO:0000072 | Awns were ranked in five categories, 1-5 scale, from no awns to awns over 1 cm in length. |
Days to heading | TO:0000137 | Days to heading was evaluated on 10 plants per row as the number of days from seeding to until 10% of the panicles had emerged. |
Germination rate | TO:0000430 | Percent germination was determined using 30 kernels that had been harvested 30 days after the panicle had flowered, dried at 45 °C for 4 days, soaked in water for 2 days, and then transferred to moist filter paper in closed petri dishes for 4 days. |
Grain shattering | TO:0000473 | Shattering was estimated using five categories, 1-5 scale, ranging from non-shattering (seeds are difficult to pull off the panicle) to highly shattering (seeds fall freely without touching the panicle) at the time of harvest. |
Grain yield per plant | TO:0000449 | Yield per plant was determined, in grams, as the average weight of bulked harvested grain per plant from at least ten plants. |
Grains per panicle | TO:0000450 | Grains per panicle was measured as the average number of filled spikelets per panicle on the primary panicle of each plant. |
Panicle length | TO:0000040 | Panicle length was measured as the average number of centimeters from the panicle neck to the panicle tip, excluding awns. |
Panicle number | TO:0000152 | Panicles per plant was calculated as the average number of panicles per plant at harvest. |
Plant height | TO:0000207 | Plant height was measured in centimeters from the soil surface to the tip of the tallest panicle, excluding the awns. |
Seed set percent | TO:0000455 | Percent seed set was determined as the number of filled grains per panicle divided by the total number of spikelets per panicle. |
Spikelet number | TO:0000456 | Spikelets per panicle was calculated as the average total number of spikelets (florets) per panicle measured on the primary panicle of each plant. |
Tiller angle | TO:0000567 | Tiller type consisted of two categories - erect tiller type and lazy tiller type (greater than 10 degrees from the vertical). |