Reference "Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson"

Reference ID

8323

Title

Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson

Source

Theoretical and applied genetics, 2003, vol. 107, pp. 479-493

Authors (9)

Thomson-M-J	Tai-T-H
McClung-A-M	Lai-X-H
Hinga-M-E	Lobos-K-B
Xu-Y	Martinez-C-P
McCouch-S-R

Abstract

An advanced backcross population between an accession of Oryza rufipogon (IRGC
105491) and the U.S. cultivar Jefferson (Oryza sativa ssp. japonica) was
developed to identify quantitative trait loci (QTLs) for yield, yield components
and morphological traits. The genetic linkage map generated for this population
consisted of 153 SSR and RFLP markers with an average interval size of 10.3 cM.
Thirteen traits were examined, nine of which were measured in multiple
environments. Seventy-six QTLs above an experiment-wise significance threshold
of P<0.01 (corresponding to an interval mapping LOD>3.6 or a composite interval
mapping LOD>3.9) were identified. For the traits measured in multiple
environments, 47% of the QTLs were detected in at least two environments. The O.
rufipogon allele was favorable for 53% of the yield and yield component QTLs,
including loci for yield, grains per panicle, panicle length, and grain weight.
Morphological traits related to the domestication process and/or weedy
characteristics, including plant height, shattering, tiller type and awns, were
found clustered on chromosomes 1 and 4. Comparisons to previous studies
involving wild x cultivated crosses revealed O. rufipogon alleles with stable
effects in multiple genetic backgrounds and environments, several of which have
not been detected in studies between Oryza sativa cultivars, indicating
potentially novel alleles from O. rufipogon. Some O. rufipogon-derived QTLs,
however, were in similar regions as previously reported QTLs from Oryza sativa
cultivars, providing evidence for conservation of these QTLs across the Oryza
genus. In addition, several QTLs for grain weight, plant height, and flowering
time were localized to putative homeologous regions in maize where QTLs for
these traits have been previously reported, supporting the hypothesis of
functional conservation of QTLs across the grasses.