Seed shattering is a primitive trait common in wild rice species. This
trait has been eliminated in the process of rice domestication. Sobrizal
et al. (1999) and Nagai et al. (2002) identified Sh3,
which is commonly found in the backcross progeny of wild species. This
is the first report of an inhibitor gene for Sh3.
In the process of developing O. glaberrima (Acc. IRGC104038) chromosomal
segment introgression lines by continuous backcrossing with O. sativa
cultivar Taichung 65 (T65) as a recurrent parent (Doi et al. 1997),
we observed segregation of shattering plants in some of the BC3F3
populations. The characteristics of shattering plants were supposed to
be caused by Sh3. Three BC3F4 populations
obtained by self-pollinating seed shattering BC3F3
plants were used for the analysis of O. glaberrima allele of Sh3.
In the population analyzed, shattering and non-shattering plants totally
segregated into 62:69 (Table 1). The degree of seed shattering was so
remarkable that we could easily differentiate shattering from non-shattering
plants. Since Sh3 was located on chromosome 4, RFLP markers tightly
linked to Sh3 were used for linkage mapping. All 62 seed shattering
plants in BC3F4 population were heterozygous at
an RFLP marker R1427. Among 69 non-shattering plants, there were
29 homozygous plants for T65 and 40 homozygous plants for O. glaberrima.
These results indicate that O. glaberrima allele of Sh3
tightly linked to R1427 caused shattering
and a recessive gene from O. glaberrima also tightly linked to
R1427 inhibited the expression of the shattering gene. Linkage
map of the genes for seed shattering and inhibitor is shown in Fig. 1.
The allele of shattering gene from O. glaberrima identified in
this study was designated as Sh3-gla because Sh3 from wild
species was previously identified in the same map position (Sobrizal et
al. 1999, Nagai et al. 2002). A recessive gene inhibiting the
expression of Sh3-gla was tentatively designated as iSh3(t)
(inhibitor for Sh3-gla). Our results indicate that O. glaberrima
still has functional Sh3 and a mutation in iSh3 locus occurred
during the process of domestication of O. glaberrima.
This study was supported by Bio-oriented Technology Research Advancement
Institution (BRAIN), Japan.
References
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