30.Tagging of the restorer gene for rice HL-type CMS using
microsatellite markers
Q. HUANG1.2, Y. HE’, R. Jin1, H. Huang1 and Y. Zhu1 1) College of Life Sciences, Wuhan University, Wuhan 430072,
China
2) College of Life Sciences, Central China Normal University,
Wuhan 430079, China
Cytoplasmic male sterility (CMS)
is the commonly used system in hybrid rice breeding. There are three main
CMS sources, that is WA, BT and HL popularly used in commercial hybrid
rice seed production. Location of the restorer genes for CMS lines with
DNA markers has been investigated during the last few years. The restorer
gene for the BT-type CMS, Rf-1, has been mapped to chromosome 10 (Akagi
et a!. 1996; Ichikawa et al. 1997). However, reports regarding the locations
of restorer genes for the WA-type CMS are inconsistent. Seven chromosomal
locations have been reported: two on chromosome 1 (Zhang eta!. 1997; Yao
eta!. 1997; Shen et a!. 1998), one on chromosome 7 (Bharaj eta!. 1995),
two on chromosome 10 (Bharaj et al. 1995; Yao eta!. 1997; Tan et al. 1998),
and four QTLs on chromosome 2, 3,4 and 5 (Zhu et a!. 1996). Our study on
the inheritance of fertility restoration of FIL-type CMS indicated that
a single dominant gene controlled the trait (Huang et a!. 1998). We determined
the location of this restorer gene using microsatellite markers.
The BC, population derived from
Congguang 4lAJIMiyang 23/Congguang 41B was used for mapping this restorer
gene. Pollen fertility was recorded at flowering time. The segregation
of fertile plants (Rfrf) and sterile plants (rfrJ) in this population was
in the ratio of 1:1.
Bulked DNA segregant analysis using
microsatellites was used to identify DNA markers associated with the restorer
gene. One hundred and fifty-nine microsatellite primers were screened for
polymorphism between the parents and between two bulks representing fertile
and sterile plants. One microsatellite primer RM258 produced polymorphic
products (Fig. 1). The microsatellite primer RM258 was then used to survey
the BC, population. Amongst seventy-five fertile plants eight plants were
recombinant and amongst ninety-eight sterile plants five plants were recombinant.
The linkage analysis with Mapmaker 3.0 revealed that the restorer gene
was linked to RM258. The genetic distance between them was 7.8cM. Therefore,
the restorer gene for the HL-type CMS designated as Rf-5, was located on
chromosome 10.
It is noteworthy that Rf-1 and
Rf-4, the restorer genes of WA-type CMS were also located in the adjacent
region on chromosome 10. We postulated that Rf-5 and Rf-4 are tightly linked,
while Rf-5 and Rf-1 are allelic. Further studies are needed to clarify
the relationships among three restorer genes.
References
Akagi, H., Y. Yokozeki, A. Inagaki, A. Nakamura and T. Fujimura,
1996. A codominant DNA marker closely linked to the rice nuclear gene,
Rf-1, identified with inter-SSR fingerprinting. Genome 39: 1205-1209.
Bharaj, T.S., S.S. Virmani and G.S. Khush, 1995. Chromosome
location of fertility restoring genes for “wild abortive” cytoplasmic male
sterility using primary trisomics in rice. Euphytica 83: 169-173.
Huang, Q., Y.Q. He and Y.G. Zhu, 1998. Genetics of fertility
restoration of “HL” type cytoplasmic male sterility in rice (Oryza saliva
L.). ABSTRACTS of 18th Intl. Congress of Genetics, p. 273.
Ichikawa, N., N. Kishimoto,A. Inagaki, A. Nakamura, Y. Koshino,
Y. Yokozeki, M. Oka, S. Samoto, H. Akagi, K. Higo, Ch. Sbinjyo, T. Fujimura
and H. Shimada, 1997. A rapid PCR-aided selection of a rice line containing
the Rf-1 gene which is involved in restoration of the cytoplasmic male
sterility. Mol. Breed. 3:
195-202.
Shen, Y., Z.Q. Guang, J. Lu, .LY. Zhuang, K.L. Zheng, MW.
Gao and X.M. Wang, 1998. Linkage analysis of a fertility restoring mutant
generated form CMS rice. Theor. Appi. Genet. 97: 261-266.
Tan, X.L., A. Vanavichit, S. Amornsilpa and S. Trangoorung,
1998. Genetic analysis of rice CMS-WA fertility restoration based on QTL
mapping. Theor. Appl. Genet. 97:994-999.
Yao, F.Y., C.G. Xu, S.B. Yu,J.X. Li, Y.J. Gao, X.H. Li and
Q. Zhang, 1997. Mapping and genetic analysis of two fertility restorer
loci in the wild-abortive cytoplasmic male sterility system of rice (Oryza
sativa L.). Euphytica 98: 183-187.
Zhang, 0., T.S. Bharaj, S.S. Virmani and N. Huang, 1997.
Mapping of the Rf-3 nuclear fertility restoring gene for WA cytoplasmic
male sterility in rice using RAPD and RFLP markers. Theor. App!. Genet.
94: 27-
33.
Zhu, L., C. Lu, P. Li, L. Shen, Y. Xu, P. He andY. Chen,
1996. Using doubled haploid populations of rice for quantitative trait
locus mapping. Rice Genetics Ill, p. 631-634. IRRI, Manila, Philippines.
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