D. 
Research Notes 
I. 
Varietal Differentiation and Evolution 
1. Isozyme classification of Taiwan and Yunnan rice Germplasm of China
S.X. TANG and G.S. KHUSH
International Rice Research Institute, P.O. Box 933, Manila, Philippines

 
Isozyme markers have been extensively used to classify rice germplasm into different varietal groups based allelic association at different isozyme loci (Pgi-I, Pgi-2, Amp-3, Amp-2 and Amp-I). Germplasm classification is helpful to breeders in choosing parents for making crosses and for genetic analysis. We analyzed 2131 rice varieties from Taiwan and Yunnan provinces of China. These varieties were classified into different isozymic groups according to the method Glaszman’s classification (1987).
The results of isozyme analysis showed that 98% of the germplasm from these provinces belongs to only two groups: indica (I) and japonica (VI). In Taiwan and Yunnan, 46.1% and 50.1% of rice germplasm belong to indica, and 51.8% and 48.2% to japonica, respectively. Thus, the distribution of varieties from Taiwan and Yunnan into two groups was similar although the environment and rice growing conditions are quite different. Only a few of varieties were classified into group II and V. Thirty six varieties were intermediate types (“0”) which could not be classified into any of the groups.

 
Table 1. Isozyme classification of Taiwan and Yunnan rice germplasm of China
Province
Varieties
analyzed
   
Varietie
s in differ
ent groups
   
   
I
II
III
IV
V
VT
VII
Taiwan
1604
740
2
0
0
1
830
31
 
%
46.1
0.1
0
0
0.1
51.8
1.9
Yunnan
527
264
3
0
0
1
254
5
 
%
50.1
0.6
0
0
0.2
48.2
0.9
Total
2131
1004
5
0
0
2
1084
36
 
%
47.1
0.2
0
0
0.1
50.9
1.7
References
Glaszmann, J.C., 1987. Isozymes and classification of Asian rice varieties. Theor. Appi. Gent. 74: 2 1-30.
Malik, 
S.S. and G.S. Khush, 1996. Isozyme classification of Taiwan and Yunnan germplasm of China. RGN 
13: 43-44.