D. Research Notes
1. Varietal Differentiation and Evolution
Y.C. cho1 , G.B. gregorio2, K.H. kang1, S.N. ahn1, D.S. brar2 and H.P. moon12) International Rice Research Institute, P.O. Box 933, 1099 Manila, Philippines
AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA. AFLP analysis was carried out by using the method of Vos et al. ( 1996) with some modifications and EcoRI/MseI restriction enzyme combination (Cho et al. 1997). The number of bands for each primer pair ranged from 34 to 92 with an average of 68.3 per reaction and the polymorphic bands from 10 to 39 with an average of 21.4. These results were similar to those of Mackill et al. (1995) and Vos et al. (1996). Genetic similarities among 27 japonica rice cultivars by Nei's formular (1987) were 0.819-0.986. Cluster analysis was performed using UPGMA (Sokal and Michener 1958) based on AFLP polymorphisms (Fig. 1). All cultivars revealed a distinct fingerprint at least within 84.5% genetic similarity. Two Korean cultivars, Sobaegbyeo and Odaebyeo derived from the cross Akitsuho/Fuji 269 of Japanese cultivars, clustered together with their parents. Samnambyeo and Hwaseongbyeo also clustered with their parents, Fuji 280 and BL 1. High quality cultivar Ilpumbyeo grouped with its parent Inabawase, and Japanese high grain quality cultivars, Koshihikari and Kinuhikari. Two Korean cultivars (Jinmibyeo, Tamjinbyeo) and three U.S. cultivars (S-202, M-202, M-401) were weakly clustered with the main group, respectively.
The first breeding goal of rice in Korea has been changed to grain quality together with the elevation of the living standard since the middle of 1980s. During that time, about one hundred japonica rice cultivars have been developed for farmers in Korea. Most Korean japonica commercial rice cultivars were developed from the crosses among some japonica high quality cultivars of Japan, because Korean is similar to Japanese for the liking of rice grain quality (Table 1). So, genetic diversity of modern Korean japonica rice cultivars has been reduced due to intensive breeding efforts to develop the varieties of
Table 1. Cross combinations of commercial japonica rice cultivars of high quality cultivated in Korea | ||
Varieties | Cross combinations | Remarks |
Sobaegbyeo | Akitsuho/Fuji 269 | SR5204-37-1 (Suweon 304) |
Odaebyeo | Akitsuho/Fuji 269 | SR5204-39-8-2 (Suweon 303) |
Samnambyeo | Fuji 280/BL t | Suweon 295 |
llpumbyeo | S.295-sv3/lnabawase | " " : Fuji 280/BL 1 |
Hwaseongbyeo | Aichi 37/Samnambyeo | F1 anther culture |
Jinmibyeo | lnabawase/SR4048-5-4-6 | SR4048 : BL7/Nongbaeg |
Cheonmabyeo | BL 7/Nongbaeg | SR4048-5-4-4-1-3-2 |
Jinbubyeo | Fukuhikari/Hokuriku 109 | Flikuhikari : Koshihikari/Fukunishiki |
Hoi<uriku109: Todorokiwase/Fuji170 | ||
//lnabawase | ||
Donaiinbyeo | Kinmaze/Nagdongbyeo | - |
//Sadominori | ||
Seomiinbyeo | Milyang 20/Asominori | - |
Tamjinbyeo | Milyang 20/Asominori | HR 769 : Palgeum/TN 1 |
//HR769/Asominori | //Palgweng/TN 1 |
Ahn, S.N., H.W. Park, H.C. Clioi and H.P. Moon. 1996. Fingerprinting of japonica rice cultivars using RAPD
diversity analysis of 48 commercial varieties in rice (0ryza sativa L.) based on AFLP. Korean J. Breed.
Mackill, D.J., Z. Zhang and E.D. Redona, 1995. Comparison of AFLP, microsatellite and RAPD marker poly
morphism in rice. RGN 12: 245-248.
Nei, M. 1987. Molecular Evolutionary Genetics. Columbia Univ. Press, NY, ppl06-107.
Sokal, R.R. and C.D. Michener, 1958. A statistical method evaluating systematic relationships. Univ. Kansas
32:1113-1118.