Two planthopper species, whitebacked planthopper (WBPH), Sogatella
furcifera Horvath and brown planthopper (BPH), Nilaparvata lugens
Stal are serious insect pests of rice (Oryza sativa L.). The rice
ovicidal response to WBPH and BPH is characterized by the formation of
watery lesion, which results in the death of their eggs at ovipositional
site (Suzuki et al. 1996, Kiyonaga et al. 1997). In this
study, quantitative trait loci (QTLs) for rice ovicidal response to WBPH
and BPH were mapped on an RFLP linkage map.
A set of 98 backcrossed inbred lines (BILs) derived from Nipponbare(Japonica)/Kasalath
(Indica)//Nipponbare (Lin et al. 1998) was used to phenotype the
rice ovicidal response to WBPH and BPH. The WBPH and BPH populations originated
from adults collected from the paddy fields of Kyushu National Agricultural
Experiment Station in Chikugo, Fukuoka, in 1989. These populations were
maintained and used for infestation. Eight weeks after seeding, a single
plant of each BIL in a 545ml plastic cup was infested with 10 to 12 reared
gravid females for two days. At three days after removing the insects,
each individual was phenotyped for grade of watery lesions (GWL) and egg
mortality (EM) to evaluate ovicidal response following Yamasaki et
al. (2000). The QTL analyses for GWL and EM were conducted by composite
interval mapping as recombinant inbred line mode in QTL Cartographer v1.13
model 6 (Basten et al. 1998). To reduce type II errors, we set
a LOD score of 1.6 as the threshold for declaring a QTL present (Yamasaki
et al. 2000).
Nipponbare showed high ovicidal response whereas Kasalath showed low ovicidal
response. The BILs for the ovicidal response to WBPH showed continuous
distributions (Fig. 1). Transgressive resistant individuals for EM beyond
the value of Nipponbare were observed. A total of six QTLs for the ovicidal
response to WBPH were detected on chromosomes 3, 6, 7, 8 and 10 (Table
1). A major QTL was identified on chromosome 6 and accounted for 33.8%
(GWL) and 46.4% (EM) of the phenotypic variance. The alleles from Nipponbare
on chromosomes 6, 7, 8 and 10 increased values of the ovicidal response
whereas the alleles from Kasalath on chromosomes 3 and 7 positively contributed
to the ovicidal response. The accumulation of the positive QTLs alleles
from both parents might explain the genetic basis of the transgressive
segregation for EM.
The frequency distributions of the ovicidal response to BPH seemed to
be bimodal (Fig. 1). Three QTLs were detected on chromosomes 2, 5 and
6 (Table 1). The chromosome 6 QTL explained 84.7% of phenotypic variance
in GWL and EM, suggesting that this QTL may be responsible for the bimodal
distributions. The alleles from Nipponbare at three QTLs contributed the
positive values of the ovicidal response.
It was concluded that the Nipponbare allele at the chromosome 6 QTL was
most important for the ovicidal response to both planthoppers. This QTL
corresponds to ovicidal gene (Ovc), which was identified
using a nearly isogenic population derived from a Japonica-Indica cross
of Asominori/IR24 (Yamasaki et al. 2003). The Asominori allele
at Ovc was essential for the ovicidal response to BPH as well as
to WBPH (Yamasaki et al. 2000). Thus, Japonica alleles at Ovc
in Asominori and Nipponbare were crucial for the ovicidal response to
both planthoppers. Except Ovc, no common QTL was found between
the reports using recombi-
nant inbred lines derived from Asominori/IR24 (Yamasaki et al.
1999, 2000) and present study using BILs derived from Nipponbare/Kasalath//Nipponbare.
There might be differences among genetic effect of parental alleles at
the QTLs.
Acknowledgements
We are grateful to Drs. K. Sogawa and Y. Suzuki for providing insects
and helpful comments. We also thank Dr. M. Yano for providing the plant
materials.
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