Vol. 20 >B. Research Notes>II. Varietal differentiation and evolution |
3. | Microsatellites as diagnostic markers for differentiating Indica and Japonica subspecies of Asian rice (Oryza sativa L.) |
C.N. NEERAJA , S. MALATHI and E.A. SIDDIQ Directorate of Rice Research, Rajendranagar, Hyderabad, 500030 India |
Sub specific classification of Asian rice (Oryza sativa L.) into
indica and japonica has always been a subject of interest
althrough for rice breeders and geneticists. The two subspecies differing
in a large array of morphological and physiological characteristics can
be differentiated using isozyme and DNA markers (Oka, 1988; Glaszmann,
1987; Zhang et al. 1992; Ni et al. 2002). From practical
standpoint, it is desirable to have markers that would enable easy identification
of two subspecies. Keeping this in view, the present study was undertaken
with six genotypes each of indica (IR8, T(N)1, IR36, IR20, Jaya and Khodawkmali)
and japonica (Yamadanshiki, Hinohikari, Koshihikari, Taichung 65,
Moroberekan and Azucena) using 375 microsatellite primers covering the
entire genome. PCR conditions and profile were according to Temnykh et
al. (2000). The PCR products were analyzed on 3% agarose and stained
with ethidium bromide. variation than japonica rices in conformity with
earlier reports. The primer RM455 only was found to be associated with
Est-9 isozyme allele, which was earlier reported to differentiate
the two subspecies of O.sativa. |
Vol. 20 >B. Research Notes>II. Varietal differentiation and evolution |