Vol. 20 >B. Research Notes>IV. Genetics of physiological traits and others |
18. | Gene mapping and immunocytochemistry of rice glup6 mutant |
M. SATO, D. ISHIHARA, H.D. TIAN, Y. TAKEMOTO, T. KUMAMARU,
and H. SATOH Plant Genetics Laboratory, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, 812-8581 Japan |
The glutelin precursor (glup) mutant genes have been genetically
characterized and found that there are 9 different loci, esp2 and
Glup1 to glup8 (Kumamaru et al. 1988, Satoh et
al. 1994, 1999, Tian et al. 2001). Recently genetic analysis
indicated that glup6 independent of other glup genes and
glup6 was epistatic to genes for Glup1, glup2, glup3
and Glup5 , hypostatic to esp2 gene (Tian et al.
2001) and additive by glup4 gene. These observation suggest that the product of glup6 gene involves
in segregation of proglutelins and prolamins within ER lumen. However,
the chromosome location of glup6 was not known. In this study linkage
analysis by using PCR marker was carried out and it revealed that glup6
was located on chromosome 3. Further, to identify and isolate the key
gene involved in glup6 mutation, we constructed the linkage map
of Glup6 gene on chromosome 3. A glup6 mutant line, EM939,
induced from a japonica rice variety Taichung65 by MNU treatment,
was crossed with an indica rice variety Kasalath. Method of screening
glup6 homozygous was refered to Sato et al. (2003). F2
analysis using 41 glup6 homozygous F2 plants showed
that the glup6 gene was mapped within 10.4 cM region between PCR
markers, R10784 and R216 on Chromosome 3 (Fig. 2). Institution (BRAIN), Japan. |
Vol. 20 >B. Research Notes>IV. Genetics of physiological traits and others |