Y. tsuchimine 1, Y. miyashita1, R. isikawa1, M. senda2, S. akada2, T. harada1 and M. niizeki1
1) Faculty of Agriculture. Hirosaki University, Hirosaki, 036 Japan
2) Gene Research Center, Hirosaki University, Hirosaki, 036 Japan
Southern hybridization analysis was carried out using ten related progenies originating from Acc435. Several kinds of RFLP patterns were found (Fig. 1). As the Acc435 did not show any RT-PCR product of ORF in Rmu1 with cDNA templates from mature leaves, this mutability within the strain may be kept at lower level than usual state. The mutability would be increased by genomic shock when crossed with Japonica strains ir which abundant RT-PCR products of ORF in Rmu1 has been known (Ishikawa, unpublished data).
Several kinds of hybrid dysgenesis have been known in
Drosophila such as P-M hybrid dysgenesis caused by re-activation
of transposable elements. And also in maize, high mutability was gained
when mutable strains carrying active Mutator were crossed
130 Rice Genetics Newsletter Vol. 14
Fig. 1. RFLPs detected in self-pollinated progeny of Acc435. Genomic DNA was digested with Xho I (lanes 1 to 10) and Xmn I (lanes 11 to 12). Blot was hybridized with TNP domain of Rmu1. Lanes 1 to 10 and lanes 11 to 20 were corresponding different families in the progeny of single Acc435 accession.