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expand all sections collapse all sections  Oryza sativa QTL "CQE68" (photosynthetic ability)
QTL Accession ID CQE68
Species Oryza sativa (Rice) [ GR_tax:013681 ]
Trait Symbol PHOTOSYNAB
Trait Name photosynthetic ability
Published Symbol  
Trait Synonym(s) photosynthesis ability
Trait Category Biochemical
Chromosome 8
Comments Photosynthetic ability was estimated from Rubisco content at 5 days after heading divided by leaf area. Rubisco content was determined by subjecting the soluble proteins (2 µg) to SDS-PAGE using 12.5% (w/v) gels containing 0.1% (w/v) SDS, and the gels were stained with Coomassie brilliant blue. The intensity of the band corresponding to Rubisco was measured with an image analyzer (Umax Technologies, Calif.). The area of the flag leaf was measured with a leaf area meter (Li-Cor, Neb.), and leaves were oven-dried at 80°C for 2 days and weighed. Phenotypic effect contributed by Kasalath allele. The means of ten replications per line were used in the data analysis. Chromosomal locations of putative QTLs were determined by single-factor analysis (one-way ANOVA) with the general linear model procedure of QGENE. Two-way ANOVAs for epistatic interaction were also carried out with QGENE at Pd0.01 threshold. The tests were done only with marker loci linked to QTLs with a single-factor P of d0.01 to reduce the likelihood of committing a type-I error.
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